Antibody-Antigen Interactions
MetaSPR Auto is an accurate automatic and affordable binding affinity and kinetics analyzer for a wide variety of applications.
Antibody-Antigen Interactions
Antibody-Antigen Interactions

Ligand：Trop2 (45 kD)

Analyte：Sacituzumab govitecan (160 kD)

kon = 1.83×105 M-1 s-1

koff = 6.93×10-5 s-1

kD =0.78 nM

Results:

·Yes/No Binding

·kon and koff (kinetics) and KD (affinity)

·Crude antibody titer

·Epitope Binning

·Specificity

Applications:

·Protein interaction analysis

·Crude cell lysate analysis

·Antibody quantification and QC

·Ranking and Screening

·Drug discovery

CD32a

Ligand：CD32a

Analyte：Tocilizumab

kon = 2.13×103 M-1 s1

koff = 2.59×10-2 s-1

kD =12.2 μM

Results:

·Yes/No Binding

·kon and koff (kinetics) and KD (affinity)

·Crude antibody titer

·Epitope Binning

·Specificity

Applications:

·Protein interaction analysis

·Crude cell lysate analysis

·Antibody quantification and QC

·Ranking and Screening

·Drug discovery